Ectomycorrhizal inoculation of Douglas-fir nursery stock with commercially produced inoculum

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Statementby Ling-Ling L. Hung.
The Physical Object
Pagination[13], 103 leaves, bound :
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Open LibraryOL14265552M

Ectomycorrhizal inoculation of Douglas-fir container-grown seedlings succeeded with coninercially produced Laccaria laccata and Hebeloma crustuliniforme in an research greenhouse and/or commercial nurseries. However, Pisolithus tinctorius inoculum was less effective and the most sensitive to inoculum Author: Ling-ling L.

Hung. Commercially produced vegetative inocula of Laccaria laccata and Hebeloma crustuliniforme successfully formed ectomycorrhizae with Douglas-fir transplanted container (plug+1) seedlings. After months in containers, 83% and 90%, respectively, of short roots were mycorrhizal.

laccata- or H. crustuliniforme-inoculated seedlings had significantly more mycorrhizal and total short Cited by:   Douglas fir (Pseudotsuga menziesii) seedlings in two bare-root forest nurseries were inoculated with the ectomycorrhizal fungus Laccaria laccata, together or not with one of five mycorrhization helper bacteria isolated from L.

laccata sporocarps or mycorrhizas and previously selected by in vitro and glasshouse screenings. With the most efficient MHB isolates, when compared to the Cited by: Ectomycorrhizal inoculation of Douglas-fir nursery stock with commercially produced inoculum Isolates that grow well over a broad pH range would be preferred for inoculation in tree nurseries.\ud Ectomycorrhizal inoculation of Douglas-fir container-grown seedlings succeeded with coninercially produced Laccaria laccata and\ud Hebeloma.

We inoculated disinfected soil at two Douglas-fir bareroot forest nurseries with three doses (8×10 5, 8×10 7 and 8×10 9 cfu m −2) of the rifampin-resistant mycorrhiza helper bacterium Pseudomonas fluorescens strain BBc6R8 and the ectomycorrhizal fungus Laccaria bicolor strain SN.

In one of the two nurseries, two doses of fungal inoculum (50 and mg m −2 dry weight Cited by: Dougl. ex Loud.) and Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) (Molina ) by similar techniques have also been successful.

Little data is available, however, on artificial inoculation with other ectomycorrhizal fungi. Trappe () suggests that ectomycorrhizal fungi in. Effects of commercially produced inoculum on container-grown Douglas-fir and ponderosa pine seedlings February Canadian Journal of Forest Research 16(4) We used an Illumina DNA sequencing approach to measure the abundance, richness and composition of ectomycorrhizal fungi on Douglas‐fir roots, and assessed differences in Douglas‐fir seedling survival and growth near to and far from forest edges with and without forest soil inoculum.

Planting Douglas‐fir seedlings near Ectomycorrhizal inoculation of Douglas-fir nursery stock with commercially produced inoculum book edges. In this study, the effect of pre-sowing inoculation of pure peat and peat+perlite mixtures ( andv:v) with an ectomycorrhizal mycelium-bead inoculum prepared in the laboratory and.

22 Forest Nursery Pests or the nutrients.

Details Ectomycorrhizal inoculation of Douglas-fir nursery stock with commercially produced inoculum FB2

Managers interested in using or testing a particular mycorrhizal species may have to special order a single species product. Some nurseries produce their own mycorrhizal inoculum.

Ectomycorrhizal inoculum is made. Shannon M Hagerman, Stacey M Sakakibara, Daniel M Durall, The potential for woody understory plants to provide refuge for ectomycorrhizal inoculum at an interior Douglas-fir forest after clear-cut logging, Canadian Journal of Forest Research, /x, 31, 4, (), ().

Because of its ecological adaptability, however, nursery soil inoculation with Pisolithus is usually successful. Techniques have been developed for mass production of Pisolithus inoculum and inoculation of both bare-root and container-grown seedlings (Marx et al.

; Marx et al. Mycorrhizae inoculation of Douglas-fir seedlings is a common practice in commercial nurseries [33,34]. However, commercial nursery treatments, including frequent addition of fertilizer and water, may not be favorable for mycorrhizae colonization of container-grown seedlings.

USDA-ARS affiliated researchers tested the hypothesis that: (1) IAA concentration of mycorrhizal conifer roots is influenced by the in vitro (IAA and ethylene) production capacity of their associated ectomycorrhizal fungi; and (2) increased concentrations of root IAA cause an increase in root growth resulting in improved seedling growth and survival.

Medicago truncatula handbook version November Inoculation and Growth with Mycorrhizal Fungi Mireille Chabaud1, Maria Harrison2, Fernanda de Carvalho-Niebel1, Guillaume Bécard3 and David G. Barker1. 1Laboratoire des Interactions Plantes-Microorganismes, INRA-CNRS, BPCastanet Tolosan Cedex, France.

2Boyce Thompson Institute for Plant Research, Tower Road. Hagerman SM, Durall DM () Ectomycorrhizal colonization of greenhouse-grown Douglas-fir (Pseudotsuga menziesii) seedlings by inoculum associated with the roots of refuge plants sampled from a Douglas-fir forest in the southern interior of British Columbia.

Can J Bot – An ectomycorrhiza (from Greek ἐκτός ektos, "outside", μύκης mykes, "fungus", and ῥίζα rhiza, "root"; pl. ectomycorrhizas or ectomycorrhizae, abbreviated EcM) is a form of symbiotic relationship that occurs between a fungal symbiont, or mycobiont, and the roots of various plant mycobiont is often from the phyla Basidiomycota and Ascomycota, and more rarely from the.

INTRODUCTION. The ectomycorrhizal symbiosis is an important factor for the survival and growth of many forest trees. The predominance of positive effects of this symbiosis in the establishment and growth of forest plants demonstrates that ectomycorrhizal inoculation is a valuable tool for plant production in forestry (7, 19).Species of the ectomycorrhizal genus Pisolithus are among those most.

Comparative effects of the soil microflora on ectomycorrhizal inoculation of conifer seedlings B. Mcafee Centre de Recherches en Biologie Forestière, Faculté de Forestérie et de Géodésie, Université Laval, Ste‐Foy, Québec, Canada G1K 7P4.

All sites were harvested prior to and planted with Douglas-fir. In the fall ofthe sites were de-stumped to reduce the inoculum load of A.

ostoyae and, as a result, the previously planted seedlings were also removed. Download Extension Note 19 PDF file ( KB) To view this document you need the current version of.

Selection of Fungi for Ectomycorrhizal Inoculation in Nurseries J M Trappe Annual Review of Phytopathology Role of Mycorrhizae in Root Disease B Zak Annual Review of Phytopathology Factors Affecting the Efficacy of Fungicides in Soil D E Munnecke Annual Review of Phytopathology Fungicide Usage on Deciduous Fruit Trees F H Lewis, and and K D Hickey.

To propagate the inoculum, the cultures were mixed with a blender every 3–4 weeks and transferred into new Erlenmeyer flasks containing sterile growing medium. The inoculum was homogenized in a grinder immediately before application.

Experimental design. A pot experiment was carried out at a greenhouse for 6 months from 20 April to 20 October.

Intersite variation in ectomycorrhizal (ECM) inoculum potential in soils from 16 sites located in arid subalpine areas of the White Mountains of California was quantified.

The study sites included valleys dominated by big sagebrush (Artemisia tridentata Nutt.) and mountainsides dominated by ancient Great Basin bristlecone pine (Pinus longaeva.

The inoculum used for mycorrhizal inoculation was obtained from these cultures, and prepared as described in the study by Brulé and colleagues ().

Description Ectomycorrhizal inoculation of Douglas-fir nursery stock with commercially produced inoculum PDF

Two tree species were inoculated with L. bicolor SN mycelium: Douglas fir (Pseudotsuga menziensii) and oak (Quercus robur). [In preparation]. Douglas-fir seedling performance following basidiospore inoculation: fungal effectiveness and genotypic variation.

Amaranthus, M. P.; Perry, D. The effect of soil transfer on ectomycorrhiza formation and the survival and growth of conifer seedlings on old, non reforested clear-cuts. Canadian Journal of Forest Research. Ectomycorrhizal diversity within the soil may buffer the impact of disturbance on forest sites.

Download Ectomycorrhizal inoculation of Douglas-fir nursery stock with commercially produced inoculum EPUB

Disturbance did not reduce ectomycorrhiza formation on Douglas-fir seedlings grown in soil from extremely productive sites in the Oregon Cascades, where diversity of ectomycorrhizal fungi is high (Pilz and Perry ; Schoenberger and Perry ). Low ectomycorrhizal inoculum potential and diversity from soils in and near ancient forests of bristlecone pine (Pinus longaeva) M.I.

Bidartondo, J. Baar, and T.D. Bruns Abstract: Intersite variation in ectomycorrhizal (ECM) inoculum potential in soils from 16 sites located in arid subal-pine areas of the White Mountains of California was.

Control seedlings, produced without artificial inoculation on fumigated substrates, and even seedlings inoculated with selected fungal species are frequently colonized by indigenous nursery fungi (Kropáček and Cudlín, ; Marx, ; Perrin et al., ; Selosse et al., ) which may be one of the main reasons for the failure or low.

Inoculation of forest seedlings with mycorrhizal fungi and rhizobacteria can improve the morphological and physiological qualities of plants, especially those used for regeneration of arid areas. In this paper, under standard nursery conditions, Aleppo pine seedlings were inoculated with Pseudomonas fluorescens CECT rhizobacteria.

Some of these seedlings were also inoculated with the. The survival and development of two inoculant ectomycorrhizal fungi (Hebeloma westraliense Bough.

Tom. and Mal. and Setchelliogaster sp. nov.) on roots of outplanted Eucalyptus globulus Labill. was examined at two expasture field sites in the south-west of Western Australia. Site 1 was a gravelly yellow duplex soil, and Site 2 was a yellow sandy earth.

responses to inoculation depend on the EMF isolate used as inoculum. Although commercially-produced inocula of certain types of mycorrhizal fungi (arbuscular and ectomycorrhizal fungi) are readily available to horticulturists,little is known about the benefits,if any,of inoculation with EMF on cutting propagation in woody.wikiHow: How-to instructions you can trust.The purpose of this research is to develop improved ectomycorrhizal mixtures for use in reforestation efforts on normal soil as well as in regions that have unfavorable conditions, such as sandy, drought-affected, and reclaimed areas.

The aim is to identify suitable host-fungus pairs that have the highest probability of survival and exhibit efficient growth in problem sites such as abandoned.